PerPETuate

Home
PerPETuate

Pet DNA and Cell Banking Systems Cell culturing,
2. DNA preservation,
3. Genotyping. All of these services are included in our lump sum fee.

PERPETUATE's packaged service provides the pet owner with the following three biotechnologies performed on their pet's cell biopsy:

1. Further description of these services can be found below. If you are an international client, please view the information on our website (http://perpetuate.net/services/international_pets.php). Cell Culturing and DNA Preservation
DNA preservation begins with the p

ainless collection of tissue samples from your pet by a veterinarian. Skin biopsies are used to collect tissue samples. Your pet will feel no pain as the procedure is done under local or general anesthesia. These tissue samples are then shipped on ice gel packs by overnight courier to the laboratory in Massachusetts where they are cultured. Culturing involves growing and isolating cells from the tissue samples until a cell line of several million fibroblast cells is established. Once a cell line with your pet’s DNA is established, the cell line is frozen by a graduated process to extremely low temperatures then stored in liquid nitrogen in PERPETUATE's Bio-Kennels. Culturing and preserving DNA takes three to six weeks to complete, then cells can be preserved in liquid nitrogen for decades. Genotyping
In most cases, a genotype or ’DNA signature’ will also be developed for your pet. With this DNA signature, you will know that this is your pet, and that cells are preserved from your pet for its future clones. Genotypes are produced from buccal samples collected by your veterinarian or from cells grown in the laboratory. Buccal samples are collected by swabbing the gums of your pet with a small brush, after which, it takes about two months to complete a DNA signature. Genotypes are produced by the independent Veterinary Genetics Laboratory at the University of California in Davis, California. Canine and Feline Genotypes
When the culturing and genotyping procedures are complete, you will be presented with a PERPETUATE DNA Signature and Certificate

Our ultimate goal is to add pet cloning to the PERPETUATE biotechnology portfolio as soon as possible so you can get your special pet once again.

11/11/2025

Wai'iki Polo, beautiful day but windy and brrrr!

01/07/2018

CHARLOT’S CLONES

One year after receiving Charlot's four clones their owner offers these captivating observations:

“Physically, all four dogs are stronger and healthier than Charlot. Their knees feel pretty solid whereas Charlot’s were rubbery. Each has a different black spot on the white body. Faces are more symmetrically marked than Charlot’s face. All like Charlot are hair machines!

All four are lap dogs, they like nothing better. Each is affectionate. They couldn’t be nicer in that regard. Now, as they are reaching maturity, we see intelligent understanding in their eyes. They listen to speech. Very smart and comprehend a lot. Just like the donor Charlot.

Neighbors used to call Charlot and his brother "the opera singers" because they'd howl together occasionally. Now, all four dogs briefly howl together at least once or twice a day.

All four dogs have aspects of Charlot’s personality, but different aspects and not in the same combination. I think it’s true that clones are twins look-alike individuals having their own souls and personalities.

These dogs are smart and lovable. I love the cloned dogs, they’re my children. Thank you for making these miracles happen.”

06/04/2018

KEN AND HENRY CELEBRATE THEIR 5TH BIRTHDAY

Melvin was the best dog that Dr. Phillip and Paula Dupont ever owned. When Melvin, a mixture of Catahoula and Doberman, turned nine and began to age in 2012, the Duponts decided to preserve his genetics.

Dr. Dupont biopsied two small pieces of Melvin’s skin and sent them to PerPETuate’s laboratory. PerPETuate successfully cultured and then cryopreserved more than three million viable Melvin cells.

Phillip and Paula wanted to use these cells to clone Melvin however they had two concerns. First, the cost to clone a dog was $70,000! Second, only one biotech company was able to clone dogs, the Sooam Biotech Research Foundation in South Korea.

The Duponts justified the cost by the fact that Melvin was an exceptional dog. He greeted visitors to the Dupont veterinary clinic with barking hellos and he had an uncanny ability to understand English. Most important Melvin provided Paula and Phillip with great pleasure. Besides, they reasoned $70,000 was less than the cost of a new Land Cruiser!

Next, the Duponts visited Sooam in South Korea. Being impressed by the biotech staff, laboratory and facilities and by how well the dogs were treated, they contracted Sooam to clone Melvin. In mid-2012 PerPETuate shipped some of Melvin’s cultured cells to South Korea. Sooam used these cells to produce two clones of Melvin—Ken and Henry.

In spring of 2013, Ken and Henry joined Paula, Phillip and Melvin in Louisiana. For the next two years, Melvin was a role model for his clones. When Melvin did pass away having two identical replicas helped ease the couple’s grief. The Duponts say that Ken and Henry are more like Melvin every day. “Their personalities are identical,” says Paula.

10/02/2018

PERPETUATE’S FIRST 2018 CLONE:

GUNNI, PerPETuate’s first 2018 clone, was released to her owner Mrs. Monni Must on Jan 5th by ViaGen. Gunni and Monni immediately bonded. Monni said Gunni has the same social personality as her donor—Billy Bean who is still living. They both love people and are fearless. On the flight home the whole airport took photos of Gunni and the Delta attendants passed her around during the flight.

Gunni was well received by all except her donor—Billy Bean! Immediately Billy was envious of Gunni and would like to have had her out of the house! After weeks of sensitive management Billy and Gunni are sharing space and beginning to form a close relationship.

Billy Bean and her clone Gunni are physically similar. Both for example are smaller than most Black Labradors and have very large paws. As Monni is a professional photographer we should be able to track the physical similarities and differences between Billy and Gunni.

Monni’s assessment of her clone—"Gunni is perfect!”

30/09/2017

Charlot’s clones will soon celebrate their six-month birthdays. Their owners said “they are all sweet, loveable and safely made it through hurricane Irma.” See postings from May 29 and June 5 for earlier images of these Papillion clones.

25/06/2017

CHARLOT CLONES AT 3 AND 11 WEEKS OLD.

01/03/2017

DOLLY’S 20TH ANNIVERSARY

February 22nd. was the 20th anniversary of Dolly’s cloning announcement. Dolly the sheep was born on July 5, 1996 in Scotland. She was cloned at the Roslin Institute by Drs. Keith Campbell and Ian Wilmuth.

Dolly had three mothers (one provided the egg, another the DNA and a third carried the cloned embryo to term). She was created using the technique of somatic cell nuclear transfer. She was the first clone to be produced from an adult mammal cell.

Dolly lived her entire life at the Roslin Institute in Edinburgh. There she was bred with a Welsh Mountain ram and produced six lambs in three pregnancies.

Dolly died from lung cancer on February 14, 2003. Any connection between Dolly being a clone and her early death were dismissed. First other sheep in Dolly’s flock died of the same disease as she did. Second four other Dolly clones were produced after Dolly all of which outlived her and are still living.

The linked article provides more information: http://www.economist.com/blogs/graphicdetail/2017/02/daily-chart-12

22/11/2016

PERPETUATE’S FIRST FELINE CLONES

PerPETuate’s first feline clones are more than one year old. Both clones were produced from a PerPETuate cell line at ViaGen’s cloning laboratory on September 27, 2015. The European owner, who chooses to remain unidentified and not to publicize images of his original cat, is completely satisfied with the clones. Both clones are incredibly similar physically and behaviorally to the original cat and to one another.

13/10/2016

BOB SEMEL’S CLONES
Bob Semel, a spirited Deer Chihuahua, lost his life in September 2011. Clones of Bob, produced from cells cryopreserved by PerPETuate, were born in January 2012. Bob’s clones will celebrate their 5th birthday next January.

02/08/2016

CLONED ANIMALS ARE HEALTHY AND SAFE

Twenty years after the birth of the world’s first clone—Dolly the Sheep—extensive research has proven that cloned animals are healthy, safe and age normally. These conclusions result from analyzing thirteen clones of Dolly during the last fourteen years. All of the clones including four, who are now eight years old, are free of the diseases that caused Dolly’s premature death when she was six years old.

Animal cloning is increasingly more reliable, better understood and accepted by animal owners and the public. New cloning applications like stem cell therapy, medical and behavioral R&D, preserving endangered species, reviving extinct ones and other unique prospects, bodes well for the future of animal cloning.

For more information, please see: http://www.nature.com/ncomms/2016/160726/ncomms12359/full/ncomms12359.html

30/10/2015

CLONES OF BOB SEMEL
Bob Semel’s clones are now fully grown and nearly four years old!

PerPETuate clients and friends will remember Bob Semel and his three clones. The clones were born in South Korea in January 2012 following Bob’s tragic death in August 2011. The three clones-Bob, Jim and Tom-were some of the first dogs to be produced from a PerPETuate cell line.

From the time the clones reached their Los Angeles home in March 2012, Dr. George Semel their owner continued to be amazed with how similar the clones were to Bob and to one another in terms of temperament, personality and physically. One of the three clones-Tom-was lost in a traffic accident in when he was a year old. The other two clones-Jim and Bob-are now fully grown, healthy and full of life. See video of clones in post below.

Address

Telephone

808-989-2028

Website

http://perpetuate.net/services/international_pets.php

Alerts

Be the first to know and let us send you an email when PerPETuate posts news and promotions. Your email address will not be used for any other purpose, and you can unsubscribe at any time.

Contact The Business

Send a message to PerPETuate:

Shortcuts

Want your business to be the top-listed Transport Service?

Country:

City: